Evaluation of the Purity of Plant Genetic Material of Dry Seeds and Modern Leaves of Some Local Barley (Hordeum vulgare L.) Varieties using CTAB, SDS and Mixture Extraction Solutions (CTAB and SDS)

Elmundr Abugnia; Mohamed A. Elahmar; Wagdi Ghaleb; Naser Mohamed Al. M. Abdalgader; Talal  Emhemed  Yahia

doi:10.65405/3djkpe84

المؤلفون

Elmundr Abugnia Libyan Biotechnology Research Center, Tripoli, Libya المؤلف
Mohamed A. Elahmar Libyan Biotechnology Research Center, Tripoli, Libya المؤلف
Wagdi Ghaleb Libyan Biotechnology Research Center, Tripoli, Libya المؤلف
Naser Mohamed Al. M. Abdalgader Engineering Department, Libya Open University, Tripoli, Libya المؤلف
Talal Emhemed Yahia Libyan Biotechnology Research Center, Tripoli, Libya المؤلف

DOI:

https://doi.org/10.65405/3djkpe84

الكلمات المفتاحية:

Extraction DNA, Barley Plant, Purity, Genomic DNA,

الملخص

The extract of genomic DNA from dry seeds as well as young leaves of various local Hordeum vulgare, or barley plants, the research evaluates the efficiency of three plant DNA isolation methods such as CTAB, SDS, and the integration of both. The CTAB extraction procedure effectively eliminated impurities that are linked to inhibitory chemicals, as evidenced by spectrophotometric studies. This procedure also yielded the highest DNA purity for both fresh leaves and dry seeds. At electrophoresis exposed at 70 V for 60 minutes, for DNA migration patterns, which showed intact and recoverable genomic DNA, the results validated the successful extraction of plant genetic material. The spectrophotometric experiments indicated that the CTAB protocol of extraction was successful in the removal of contaminants associated with inhibiting compounds. Furthermore, this approach resulted in the maximum DNA purity for fresh leaves and dried seeds. The research outcome in the earlier stage corroborated CTAB efficiently, which separated DNA from secondary metabolite-rich plants. Furthermore, lower purity values (about 1.30) have been obtained utilizing the combined method (CTAB + SDS), indicating the presence of residual impurities. Because of limited effectiveness in tissues containing polysaccharides as well as phenolic compounds, SDS alone yielded very low-quality DNA (purity ~1.65).

التنزيلات

تنزيل البيانات ليس متاحًا بعد.

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