Production of Probiotic Soft White Cheese Using a Mixture of Fresh Whole Cow’s Milk and Powdered Milk
DOI:
https://doi.org/10.65405/cjos.2025.798Keywords:
Fresh White cheese; Probiotic soft White Cheese; High-Yield White Cheese.Abstract
Soft white cheese is a very popular traditional dairy product in Libya and is made from fresh or pasteurized milk. There are currently few documented reports on the production of fresh Libyan white cheese using Lactic acid bacteria. Thus, this study proposed a new protocol for producing soft white cheese using a mixture of whole cow’s milk and whole milk powder, with the addition of lactic acid bacteria strains to obtain a probiotic-enriched cheese and reduce the proportion of whey as a by-product. Different ratios of whole milk powder (0, 10, 20, and 30%) were added to whole cow’s milk during cheese production. The results showed that cheese yield increased to 74.43% when 30% milk powder was used, compared to 12.23, 26.70, and 55.10% for cheeses made with 0, 10, and 20% milk powder, respectively. Sensory evaluation revealed that using 10–20% milk powder combined with lactic acid bacteria starter had no significant effect on the sensory properties of the soft cheese compared to traditional white cheese. However, excessive addition of milk powder reduced sensory acceptance among panelists. To improve yield and texture quality without negatively affecting sensory characteristics, a balanced combination of the two milk sources is recommended to produce a high-quality product that meets consumer preferences.
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Abd‐El Salam, M., & Benkerroum, N. (2006). North African brined cheeses. In A.Y. Tamime (Ed.), Brined Cheeses. (pp. 139-187). Blackwell Publishing Ltd. https://doi.org/10.1002/9780470995860.ch5
Benkerroum, N. (2013). Traditional fermented foods of North African countries: technology and food safety challenges with regard to microbiological risks. Comprehensive Reviews in Food Science and Food Safety, 12(1), 54-89. https://doi.org/10.1111/j.1541-4337.2012.00215.x
Buriti, F. C., da Rocha, J. S., Assis, E. G., & Saad, S. M. (2005). Probiotic potential of Minas fresh cheese prepared with the addition of Lactobacillus paracasei. LWT-Food Science and Technology, 38(2), 173-180. https://doi.org/10.1016/j.lwt.2004.05.012
Cárdenas, N., Calzada, J., Peirotén, Á., Jiménez, E., Escudero, R., Rodríguez, J. M., Medina, M., & Fernández, L. (2014). Development of a potential probiotic fresh cheese using two Lactobacillus salivarius strains isolated from human milk. BioMed Research International, 2014. https://doi.org/10.1155/2014/801918
Dabevska-Kostoska, M., Velickova, E., Kuzmanova, S., & Winkelhausen, E. (2015). Traditional white brined cheese as a delivery vehicle for probiotic bacterium Lactobacillus casei. Macedonian Journal of Chemistry and Chemical Engineering, 34(2), 343-350. https://doi.org/10.20450/mjcce.2015.519
Dantas, A. B., Jesus, V. F., Silva, R., Almada, C. N., Esmerino, E. A., Cappato, L. P., Silva, M. C., Raices, R. S. L., Cavalcanti, R. N., Carvalho, C. C., Sant’Ana, A. S., Bolini, H. M. A., Freitas, M. Q. & Cruz, A. G. (2016). Manufacture of probiotic Minas Frescal cheese with Lactobacillus casei Zhang. Journal of Dairy Science, 99(1), 18-30. https://doi.org/10.3168/jds.2015-9880
de Paula, C. M., dos Santos, K. M. O., Oliveira, L. S., da Silva Oliveira, J., Buriti, F. C. A., & Saad, S. M. I. (2020). Fat substitution by inulin in goat milk ice cream produced with cajá (Spondias mombin) pulp and probiotic cultures: influence on composition, texture, and acceptability among consumers of two Brazilian regions. Emirates Journal of Food and Agriculture, 140-149. https://doi.org/10.9755/ejfa.2020.v32.i2.2074
Effat, B. A., Mabrouk, A. M., Sadek, Z. I., Hussein, G. A., & Magdoub, M. N. (2020). Production of novel functional white soft cheese. Journal of Microbiology, Biotechnology and Food Sciences, 9(4), 1259-1278.
Ehsani, A., Hashemi, M., Afshari, A., & Aminzare, M. (2018). Probiotic white cheese production using coculture with Lactobacillus species isolated from traditional cheeses. Veterinary World, 11(5), 726. https://doi.org/10.14202%2Fvetworld.2018.726-730
El-Gerbi, A. M. (1998). Evaluation the quality of Libyan fresh soft white cheese manufactured locally and potential of making it from reconstituted milk. MSc Thesis, University of Tripoli, Tripoli, Libya. (In Arabic).
Gomah, N. H., Mohamed, T. H., Ahmed, H. A., & Ragab, W. S. (2019). Production of white soft cheese without whey separation. Journal of Biochemical Technology, 10(3-2019), 105-109. https://jbiochemtech.com/storage/models/article/AiQPMaJE5qyDLucEwva2i4AfQiKchsKa7zNrAjbCSkPdXaP3kiNlQExv6XN5/production-of-white-soft-cheese-without-whey-separation.pdf Accessed July 25, 2025.
Guerrero, L., Guàrdia, M. D., Xicola, J., Verbeke, W., Vanhonacker, F., Zakowska-Biemans, S., Sajdakowska, M., Sulmont-Rossé , C., Issanchou, S., Contel, M., Scalvedi, M. L., Granli, B. G. & Hersleth, M. (2009). Consumer-driven definition of traditional food products and innovation in traditional foods. A qualitative cross-cultural study. Appetite, 52(2), 345-354. https://doi.org/10.1016/j.appet.2008.11.008
Haddad, M. A., Yamani, M., & Abu-Alruz, K. (2015). Development of a Probiotic Soft White Jordanian Cheese. American-Eurasian Journal of Agricultural and Environmental Sciences. Sci, 15(7), 1382-1391. http://dx.doi.org/10.5829/idosi.aejaes.2015.15.7.12635
Hooi, R., Barbano, D. M., Bradley, R. L., Budde, D., Bulthaus, M., Chettiar, M., Lynch, J., & Reddy, R. (2004). Chapter 15 chemical and physical methods. In E. A. Arnold (Ed.), Standard methods for the examination of dairy products (Vol. 17, pp. 443–445). American Public Health Association. https://doi.org/10.2105/9 780875530024ch15
Huppertz, T., Upadhyay, V. K. Kelly, A. L. & Tamime, A.Y. (2006). Constituents and properties of milk from different species. In A.Y. Tamime (Ed.), Brined Cheeses. (pp. 1-42). Blackwell Publishing Ltd. http://dx.doi.org/10.1002/9780470995860.ch1
Irlinger, F., Helinck, S., Jany, J. L. (2017). Secondary and Adjunct Cultures. In P. L. H. McSweeney, P.F. Fox, P. D. Cotter, and D. W. Everett, (Eds.), Cheese: chemistry, physics and microbiology. Vol. 1. 4th ed. (pp. 273-300). Academic Press. London: Elsevier. https://doi.org/10.1016/B978-0-12-417012-4.00011-9
ISO 8589. (2007). Sensory analysis. General guidance for the design of test room. HIS, Geneva.
Kayagil, F., & Candan, G. (2009). Effects of starter culture combinations using isolates from traditional cheese on the quality of Turkish white cheese. International Journal of Dairy Technology, 62(3), 387-396. https://doi.org/10.1111/j.1471-0307.2009.00493.x
McSweeney, P. L. H., Ottogalli, G., Fox, P. F. (2017). Diversity and classification of cheese varieties: An Overview. In P. L. H. McSweeney, P.F. Fox, P. D. Cotter, and D. W. Everett, (Eds.), Cheese: chemistry, physics and microbiology. Vol. 1. 4th ed. (pp. 781-808). London: Elsevier. https://doi.org/10.1016/B978-0-12-417012-4.00031-4
Oliveira, M. E. G. D., Garcia, E. F., Queiroga, R. D. C. R. D. E., & Souza, E. L. D. (2012). Technological, physicochemical and sensory characteristics of a Brazilian semi-hard goat cheese (coalho) with added probiotic lactic acid bacteria. Scientia Agricola, 69, 370-379. Souza, C. H., & Saad, S. M. (2009). Viability of Lactobacillus acidophilus La-5 added solely or in co-culture with a yoghurt starter culture and implications on physico-chemical and related properties of Minas fresh cheese during storage. LWT-Food Science and Technology, 42(2), 633-640. https://doi.org/10.1016/j.lwt.2008.07.015
Ouadghiri, M., Amar, M., Vancanneyt, M., & Swings, J. (2005). Biodiversity of lactic acid bacteria in Moroccan soft white cheese (Jben). FEMS microbiology letters, 251(2), 267-271. https://doi.org/10.1016/j.femsle.2005.08.012
Samet-Bali, O., Ayadi, M. A., & Attia, H. (2009). Traditional Tunisian butter: Physicochemical and microbial characteristics and storage stability of the oil fraction. LWT-Food Science and Technology, 42(4), 899-905. https://doi.org/10.1016/j.lwt.2008.11.007
Sharif, M. K., Masoos, S. B., Hafiz, R. S., Muhammmad, N. (2017). Sensory evaluation and consumer acceptability. Faisalabad: University of Agriculture.
Souza, C. H., & Saad, S. M. (2009). Viability of Lactobacillus acidophilus La-5 added solely or in co-culture with a yoghurt starter culture and implications on physico-chemical and related properties of Minas fresh cheese during storage. LWT-Food Science and Technology, 42(2), 633-640. https://doi.org/10.1016/j.lwt.2008.07.015
Stefanovic, E., Kilcawley, K. N., Roces, C., Rea, M. C., O'Sullivan, M., Sheehan, J. J., & McAuliffe, O. (2018). Evaluation of the potential of Lactobacillus paracasei adjuncts for flavor compounds development and diversification in short-aged cheddar cheese. Frontiers in Microbiology, 9, 1506. https://doi.org/10.3389/fmicb.2018.01506
Sulieman, A. M. E., Ali, R. A. M., & Razig, K. A. A. (2012). Production and Effect of Storage in The Chemical Composition of Mozzarella Cheese. International Journal of Food Science and Nutrition Engineering, 2(3), 21-26. https://doi:10.5923/j.food.20120203.02
Yerlikaya, O., & Ozer, E. (2014). Production of probiotic fresh white cheese using co-culture with Streptococcus thermophilus. Food Science and Technology, (Campinas), 34(3), 471-477. https://doi.org/10.1590/1678-457x.6365
Zomorodi, S., Asl, A. K., Rohani, S. M. R., & Miraghaei, S. (2011). Survival of Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium bifidum in free and microencapsulated forms on Iranian white cheese produced by ultrafiltration. International Journal of Dairy Technology, 64(1), 84-91.
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