Prevalence of Helicobacter pylori Infection Detected by ELISA in Blood and Stool Samples: A Cross-Sectional Study in Al-Bayda, Libya (2024–2025)

Authors

  • Noor alhooda M. Alawkally¹, Fathia M. Senossi2 , Abeer Alawkally3 Nessren F.Mousa4 , Maree Al Douakali Ali5 , Leenah M. Al mehdaowi6 Salma M Al mehdaowi7 . & Sarah M. mehdaowi8 1,5,6,7,8 Department of Medical Laboratory, Higher Institute of Science and Technology, Suluq, Libya 2 Department of Zoology, Collage of Art and Science, Benghazi University, Libya 3 Department of Veterinary Medical Sciences, Higher Institute of Agricultural Technology, Derna, Libya 4 Department of statistics, Collage of Art and Science, Benghazi University, Libya , Author

DOI:

https://doi.org/10.65405/.v10i37.522

Keywords:

Helicobacter pylori, Epidemiology, Seroprevalence, Libya, Diagnostic Methods, Stool Antigen Test, IgG Antibodies.

Abstract

Background and Aim: Helicobacter pylori infection represents a major global health
challenge, with epidemiological patterns showing considerable geographic variation. This
investigation sought to evaluate the current status of H. pylori infection within a patient
population in Al-Bayda, Libya, focusing on infection frequency and its correlation with key
demographic variables.
Methods: This observational analysis was carried out between 2024 and 2025. The study
cohort comprised 748 individuals. Detection of H. pylori was performed using enzyme-linked
immunosorbent assay (ELISA) techniques on two sample types: serum for quantifying
specific IgG antibodies and stool for identifying bacterial antigens.
Results: Laboratory analysis identified a positive infection rate of 39.3%. The data revealed a
higher frequency of positive results among female participants (64.6%) compared to male

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Published

2025-11-25

Issue

Vol. 10 No. 37 (2025): Comprehensive Journal of Science

Section

مؤتمر

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

Prevalence of Helicobacter pylori Infection Detected by ELISA in Blood and Stool Samples: A Cross-Sectional Study in Al-Bayda, Libya (2024–2025). (2025). Comprehensive Journal of Science, 10(37), 1350-1360. https://doi.org/10.65405/.v10i37.522